Magnetic Affinity BioScience Beads are:

• Faster and more cost-effective than traditional column affinity chromatography,
• Don't require columns or fraction collectors
• Made with biotechnology-grade agarose,
• Filled with micronized magnetic particles immobilized in the gel, and
• Don't exhibit non-specific binding- like some other magnetic beads

  As a result, they have all the advantages of traditional agarose affinity methods plus the advantage of being able to be quickly localized or retrieved with a magnet. This substantially shortens each step in the affinity purification process : exposure to sample, washing off un-bound sample components and finally washing the target componet free of the Iigand on the bead.

     Affinity binding and desorption kinetics take place in a fraction of the time required for slow column elution of solute-containing liquids. For a review of these kinetics, see " Analysis of Macromolecular Interactions Using Immobilized Ligands", by Irwin Chaiken, Sergio Rose and Robert Karlsson, in Analytical Biochemistry, 201, 197-210 (1992). Using a magnetic bioseparation technique minimizes the time required for target substance recovery . Magnetic bioseparation can also avoid the unecessary dilution often introduced by typical column elution procedures.

     Unlike some magnetic beads, agarose magnetic beads exhibit no non-specific binding and are easily kept suspended with gentle mixing.

Magnetic Agarose Beads are typically used in all of the following formats:

• microtiter plate(s),
• test tube(s),
• beaker(s), and
• bioreactor(s)

     Before using magnetic glass or magnetic plastic beads from other suppliers, it's advisable to consult the following, benchmark publication and many others like it which have appeared more recently:

"Quantitation of Protein Absorbance to Glass and Plastics.." by Paul Christensen, Arne Johansson and Villy Nielsen, Journal of Immunological
Methods, 23 (1978) 23-28.

Magnetic BioScience Beads
6% agarose; 25-75 µ

Much more expensive magnetic agarose beads from alternative supplier. 6% agarose; 25-75 µ; mag: 400 X

BioScience Magnetic Bead
size range;
6% agarose; 25-75µ; mag: 400 X

Size and shape range of
magnetic agarose beads from alternative supplier;
6% agarose; 35-75µ; mag: 400 X.